? Sensitivity Delvotest® T for cow milk // plates

Detection limits for Delvotest® T plates in cow milk

The table presented below is an indicative list of the sensitivity of the Delvotest® T at control time for the most used antibiotics in the world. This list is not exhaustive. The test is sensitive to many more antibiotic drugs and sulfonamides. Values presented in this list are ppb (parts per billion) equalling μg/Kg or ng/gram.

The best sensitivity is reached when using control time (CT). To test at control time, additionally to the milk you would like to test, perform one additional test with negative control milk, when the test containing the negative control milk turns yellow read the test result of the other milk. The sensitivity of the test when using a fixed reading incubation time (3hr15) can be decreased.

Delvotest® T is not sensitive to quinolones. Quinolones & fluoroquinolones can only be detected at levels much higher than EU MRLs.

Maximum Residue Limits (MRL)

for bovine milk (ppb)

Delvotest® T Plates CCß** ppb

Antibiotic class

Antibiotic

EU

USA S/TL

CODEX

India FSSAI

Cow milk

Penicillins

Amoxicillin

4

10

4

*

2

Ampicillin

4

10

*

10

2

Cloxacillin

30

10

*

10

10

Nafcillin

30

*

*

*

3

Oxacillin

30

*

*

*

3

Penicillin G

4

5

4

*

1

Tetracyclines

Chlortetracycline

100

300

100

100

150

Doxycycline

*

*

*

*

50

Oxytetracycline

100

300

100

100

80

Tetracycline

100

300

100

100

100

Sulfonamides

Sulfadiazine

100

10

*

10

50

Sulfadimethoxine

100

10

*

*

40

Sulfadoxine

100

*

*

*

80

Sulfamethazine

100

10

25

25

125

Sulfathiazole

100

10

*

10

30

Cephalosporins

Cefalexin

100

*

*

*

30

Cefalonium

20

*

*

*

5

Cefazolin

50

*

*

*

3

Cefoperazone

50

*

*

*

20

Cefquinome

20

*

*

10

60

Ceftiofur

100

100

100

100

20

Cephapirin

60

20

*

10

5

Desacetylcephapirin

60

*

*

*

2

Desfuroylceftiofur

100

100

100

*

80

Macrolides

Erythromycin A

40

50

*

*

200

Spiramycin

200

*

200

*

2000

Tilmicosin

50

*

*

*

100

Tylosin A

50

50

100

*

35

Aminoglycosides

DH Streptomycin

200

125

200

20

800

Gentamycin

100

30

200

*

100

Neomycin B

1500

150

1500

1500

140

Streptomycin

200

*

200

20

1000

Others

Chloramphenicol

*

*

0

*

3500

Clavulanic Acid

200

*

*

*

800

Dapsone

*

*

*

*

10

Lincomycin

150

*

150

150

220

Pirlimycin

100

400

100

*

300

Rifaximin

60

*

*

*

40

Trimethoprim

50

*

*

10

120

* not listed, legislation regulation normally restricts the use: not to be used for lactating animals.

** CCꞵ detection capability is the lowest concentration where a substance can be detected with 95% probability.

Delvotest® T sensitivity listed as CCβ as determined (according to European legislation) by AFNOR validation study 2020-2021.

Legislation regulation references

EU-MRL

EC 37/2010

USA S/TL

FDA M-I-18-9 2018

CODEX

CX/MRL 2-2018

FSSAI

Compedium_Contaminants_Regulation_20_08_2020

SAQs:

  • What is the sensitivity of T plates?

  • What antibiotics do T plates detect?

  • Can Delvotest® T detect fluoroquinolones (antibiotic) in milk?

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