? Sensitivity Delvotest® SP NT for cow milk // ampoules

Detection limits for Delvotest® SPNT ampoules in cow milk

The table presented below is an indicative list of the sensitivity of the Delvotest® SPNT at control time for the most used antibiotics in the world. This list is not exhaustive. The test is sensitive to many more antibiotic drugs and sulfonamides. Values presented in this list are ppb (parts per billion) equalling μg/Kg or ng/gram.

The best sensitivity is reached when using control time (CT). To test at control time, additionally to the milk you would like to sample, perform one additional test with negative control milk, when the test containing the negative control milk turns yellow, read the test result of the sample milk. The sensitivity of the test when reading at fixed incubation time (3h for cow milk) can be decreased.

Antibiotic class

Antibiotic

Maximum Residue Limits (MRL)

for bovine milk (ppb)

Delvotest® SPNT ampoules CCß* ppb

EU

USA S/TL

CODEX

India FSSAI

Cow milk

Penicillins

Amoxicillin

4

10

4

*

2

Ampicillin

4

10

*

10

2

Cloxacillin

30

10

*

10

12

Oxacillin

30

*

*

*

4

Penicillin G

4

5

4

*

2

Tetracyclines

Chlortetracycline

100

300

100

100

300

Doxycycline

*

*

*

*

150

Oxytetracycline

100

300

100

100

300

Tetracycline

100

300

100

100

300

Sulfonamides

Sulfadiazine

100

10

*

10

65

Sulfadimethoxine

100

10

*

*

50

Sulfamethazine

100

10

25

25

75

Sulfathiazole

100

10

*

10

40

Cephalosporins

Cefalexin

100

*

*

*

45

Cefoperazone

50

*

*

*

30

Cefquinome

20

*

*

10

65

Ceftiofur (pur)*

100

100

100

100

20

Cephapirin

60

20

*

10

2

Macrolides

Erythromycin A

40

50

*

*

90

Tilmicosin

50

*

*

*

30

Tylosin A

50

50

100

*

35

Aminoglycosides

DH Streptomycin

200

125

200

20***

700

Gentamycin

100

30

200

*

90

Kanamycin

150

*

*

*

1700

Neomycin B

1500

150

1500

1500

115

Spectinomycin

200

*

200

*

2000

Others

Chloramphenicol

*

*

0

*

3000

Dapsone

*

*

*

*

2

Lincomycin

150

*

150

150

170

Rifaximin

60

*

*

*

50

Trimethoprim

50

*

*

10

160

* not listed, legislation regulation normally restricts the use: not to be used for lactating animals.

**CCß (detection capability) is the lowest concentration where substance can be detected 95% of the time.

***Ceftiofur with metabolites has a detection limit about 4 times higher.

**** for cattle 20ppb, for sheep milk 200ppb

Data based on internal and external studies collected June 2017.

SAQs:

  • What is the sensitivity of SPNT ampoules?

  • What antibiotics do SPNT ampoules detect?

Published: 10 November 2025
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